Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?

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#Question id: 22906

#Part-B Specialized Branches in Biotechnology

Which hypersensitivity reactions are T cell mediated? 

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#Question id: 19534

#Part-B Specialized Branches in Biotechnology

The equation of material balance, (Final condition + Sum of outputs) is equal to which of the following?

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#Question id: 11268

#Part-B Specialized Branches in Biotechnology

Consumption efficiency (CE) is the proportion of production at one trophic level that is eaten by, or ingested, by the next trophic level but Assimilation efficiencies (AE) is the proportion of ingested energy that is assimilated into the bloodstream. Which of the Following statement is correct?

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#Question id: 19013

#Part-A Aptitude & General Biotechnology

Ion exchange chromatography is based on the_____________

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#Question id: 4910

#Part-A Aptitude & General Biotechnology

For a trait known to be caused by a mitochondrial gene variant, there may be an occasional female that can transmit different phenotypes to her progeny on an apparently random basis. What situation might you suspect?