Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the statement is worng? Aminopetrin………s

TLS Online TPP Program

#Question id: 19929

#Part-B Specialized Branches in Biotechnology

he growth of an organism on glucose is described by the following Monod model parameters: μm = 0.5 h^-1 and Ks =0.1 g.l^-1, if the concentration of glucose in the feed is 10 g.l^-1 and the dilution rate is set to 0.4 h^-1, then the steady state concentration of glucose in the effluent will be

TLS Online TPP Program

#Question id: 19954

#Part-B Specialized Branches in Biotechnology

A continuous reactor at steady state contains 0.04 g.l^-1 of biomass and 0.02 g.l^-1 of phenol. The feed contained 0.1 g.l^-1 of phenol. The biomass yield would be

TLS Online TPP Program

#Question id: 19955

#Part-B Specialized Branches in Biotechnology

A culture system with constant environmental conditions maintained through continual provision of nutrient and removal of wastes is called __________ culture system.

TLS Online TPP Program

#Question id: 19955

#Part-A Aptitude & General Biotechnology

A culture system with constant environmental conditions maintained through continual provision of nutrient and removal of wastes is called __________ culture system.

TLS Online TPP Program

#Question id: 20043

#Part-B Specialized Branches in Biotechnology

The exponential phase may be described by the equation _______

TLS Online TPP Program

#Question id: 20044

#Part-B Specialized Branches in Biotechnology

In a batch sterilization process if ∇overall , ∇heating & ∇cooling are 32.2, 9.8 & 10.1 respectively, THEN the holding time at 121ºC of the process is (given that the specific death rate (k) of microorganisms at 121ºC is 2.54 min^-1 ):