Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

#Question id: 11133

#Part-B Specialized Branches in Biotechnology

Which of the following statements accurately describes a feature of temperature sensation by the nervous system?

#Question id: 10252

#Part-B Specialized Branches in Biotechnology

When molecular oxygen is unavailable—for example, in plant roots in flooded soils—glycolysis can be the main source of energy for cells, How?

#Question id: 433

#Part-A Aptitude & General Biotechnology

Fatty acid synthesis occurs in the ________, while fatty acid breakdown occurs in the

#Question id: 9273

#Part-B Specialized Branches in Biotechnology

In the higher excited state, chlorophyll is extremely unstable; it rapidly gives up some of its energy to the surroundings as heat, and enters the lowest excited state, the excited chlorophyll has four alternative pathways for disposing of its available energy: fluorescence, Heat loss, energy transfer, photochemistry; all process takes place in which wavelength,

#Question id: 5942

#Part-A Aptitude & General Biotechnology

On what days of March 1601 , did Sunday fall ?