Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.

Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.

II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.

TLS Online TPP Program

#Question id: 19162

#Part-A Aptitude & General Biotechnology

Which of the following is not a gel filtration chromatography?

TLS Online TPP Program

#Question id: 19163

#Part-A Aptitude & General Biotechnology

Which solvent is usually used for SEC generally use in lab?

TLS Online TPP Program

#Question id: 19164

#Part-A Aptitude & General Biotechnology

Affinity chromatography separation is based on

TLS Online TPP Program

#Question id: 19165

#Part-A Aptitude & General Biotechnology

A pull down affinity chromatography assay is used for the investigation of

TLS Online TPP Program

#Question id: 19166

#Part-A Aptitude & General Biotechnology

The first step in preparation of affinity chromatography column is _____________

TLS Online TPP Program

#Question id: 19167

#Part-A Aptitude & General Biotechnology

Acetylcholine esterase can be purified by using affinity chromatography with the use of _______ as a ligand.