#Question id: 2292
#Part-A Aptitude & General Biotechnology
which one is not function of Golgi apparatus,
#Question id: 880
#Part-B Specialized Branches in Biotechnology
The classic work of Christian Anfinsen in the 1950s on the enzyme ribonuclease revealed the relation between the amino acid sequence of a protein and its conformation. Ribonuclease is a single polypeptide chain consisting of 124 amino acid residues cross-linked by four disulfide bonds. Anfinsen’s plan was to destroy the three-dimensional structure of the enzyme and to then determine what conditions were required to restore the structure.
The critical observation of Anfinsen that the denatured ribonuclease, freed of urea and b -mercaptoethanol by dialysis;
I. The sulfhydryl groups of the denatured enzyme became reduced by air, and the enzyme spontaneously refolded into a catalytically active form.
II. These experiments showed that the information needed to specify the catalytically active structure of ribonuclease is contained in its amino acid sequence.
III. The 105 wrong pairings have been picturesquely termed “scrambled” ribonuclease.
IV. He found that scrambled ribonuclease spontaneously converted into fully active, native ribonuclease when trace amounts of b -mercaptoethanol were added to an aqueous solution of the protein.
Choose incorrect options;
#Question id: 732
#Part-B Specialized Branches in Biotechnology
Ramachandran plots can help increase the accuracy of models derived from x-ray crystallography data. The plot below was created by measuring phi, and psi for each amino acid residue in a 2.2 A resolution crystal structure. In the plot, which excludes Gly and Pro residues, selected residues (dots) are numbered 1 through 5.
Which of the following statements can be correctly stated for this plot in reference to selected residues?
A. Residues 1 and 3 are in either B sheets
B. residue 2 is in a right-handed a helix, and residue 4 is in a left-handed a helix.
C. If residue 5 were Gly, it might be in an acceptable position in the plot
#Question id: 817
#Part-A Aptitude & General Biotechnology
The aggregation of nonpolar side chains in the interior of a protein is favored by
#Question id: 11063
#Part-B Specialized Branches in Biotechnology
A man inspires 1000 ml from a spirometer. The intrapleural pressure was −4 cm H2O before inspiration and −12 cm H2O the end of inspiration. What is the compliance of the lungs?