Practice Questions

TLS Online TPP Program

#Question id: 887

Polypeptides fold rapidly by a stepwise process, how does the polypeptide chain arrive at its native conformation?

#Part-A Aptitude & General Biotechnology

proteins appear to fold in a hierarchical manner, with small local elements of structure forming and then to yield larger elements, which coalesce with other such elements to form yet larger elements and converted into its native conformation
A protein-folding pathway has been highly successful in predicting the three-dimensional structure of small proteins from their amino acid sequence atleast 156 amino acid residue that have acquired their final structure
Vander waal interaction play a significant role throughout the process, as the aggregation of nonpolar amino acid side chains provides an entropic stabilization to intermediates and, eventually, to the final folded structure
The native conformation of the protein folding by stepwise manner such as unfolded→transition state→molten globule→folded conformation by decreasing the free energy

More Questions

TLS Online TPP Program

#Question id: 19844

#Part-A Aptitude & General Biotechnology

Match the following column, which correctly represent the qPCR done by three methods and measure such as;

COLUMN I	COLUMN II
A) SYBR green	i) Annealing
B) Taqman Assay	ii) Denaturation
C) Molecular beacon	iii) pH
	iv) Amplification
	v) Extension

TLS Online TPP Program

#Question id: 19845

#Part-A Aptitude & General Biotechnology

SYBR Green qPCR method is based on____I_______ with the _____II______ relationship.

TLS Online TPP Program

#Question id: 19845

#Part-A Aptitude & General Biotechnology

SYBR Green qPCR method is based on____I_______ with the _____II______ relationship.

TLS Online TPP Program

#Question id: 19846

#Part-A Aptitude & General Biotechnology

Variants of PCR has been developed on the basis of___

TLS Online TPP Program

#Question id: 19865

#Part-A Aptitude & General Biotechnology

Match the following activity of variants in Column I with their variants of PCR in Column II given below;

COLUMN I

COLUMN II

A) Enhance efficiency

i) Multiplex PCR

B) Sequence specific amplification

ii) Hot-start PCR

C) Mutational analysis

iii) Megaprimer PCR

iv) qPCR with SYBR

v) AS-PCR

vi) RLM-RACE

TLS Online TPP Program

#Question id: 19866

#Part-A Aptitude & General Biotechnology

Which of the following variants of PCR has been devised to increase the specificity of PCR without lowering the efficiency by variation annealing temperature?