Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is:

                             

You have been put in charge of a small fermenter for growing the bacteria and aim to produce 2.5 kg of cells for inoculation of a pilot-scale reactor. What minimum amount of hexadecane substrate must be contained in your culture medium? 

TLS Online TPP Program

#Question id: 14744

#Section 5: Bioprocess Engineering and Process Biotechnology

For production of an enzyme used for synthesis of a sun protection lotion, the required kinetic data and constants are: 

vmax = 2.3 mmol.m^-3 s^-1 Km = 8.9 mmol.^-1 So = 12 mmol1- 

Find the reaction time for 95% conversion of raw material 

TLS Online TPP Program

#Question id: 14745

#Section 5: Bioprocess Engineering and Process Biotechnology

heat exchanger, one transfer unit means 

TLS Online TPP Program

#Question id: 14746

#Section 5: Bioprocess Engineering and Process Biotechnology

During cultivation of microorganism in a fermenter, various parameters are controlled by appropriate mechanism, match the following 

Probes	Mechanism
A. Thermistor	1. activation of acid/alkali group
B. Oxygen electrode	2. Activation of vegetable oil pump
C. Metal rod	3. activation of hot/cold water pump
D. pH electrode	4. increase/decrease in stirrer motor speed

Option	A	B	C	D
a)	2	3	1	4
b)	3	2	4	1
c)	1	2	4	3
d)	3	4	2	1

TLS Online TPP Program

#Question id: 14747

#Section 5: Bioprocess Engineering and Process Biotechnology

Mechanism of separation of contaminants present in air by fibrous media are. 

TLS Online TPP Program

#Question id: 14748

#Section 5: Bioprocess Engineering and Process Biotechnology

 For scaling up of a bioreactor the following parameter is assumed to be constant

TLS Online TPP Program

#Question id: 14749

#Section 5: Bioprocess Engineering and Process Biotechnology

For a turbine aeration agitation, the power consumption