Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is:

                             

You have been put in charge of a small fermenter for growing the bacteria and aim to produce 2.5 kg of cells for inoculation of a pilot-scale reactor. What minimum amount of hexadecane substrate must be contained in your culture medium? 

TLS Online TPP Program

#Question id: 12920

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The development of isothermal systems such as the ____ DNA amplification system do away with the need for a thermal cycler and have the advantage of being able to be used outside the laboratory.

TLS Online TPP Program

#Question id: 12921

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the following:-

A. Gene probe production.	i. Novel proteins
B. Genome mapping.	ii. PCR mutagenesis
C. Protein Engineering.	iii. Sequence tagged sites
D. Gene Editing.	iv. Use with blots

TLS Online TPP Program

#Question id: 12922

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

 Match the following:-

1. Ligase chain reaction.	i. Mutation detection
2. Nucleic acid sequence based amplification.	ii. Non isothermal
3. Loop mediated isothermal amplification.	iii. Viral detection
4. Branched DNA amplification.	iv. parasitic disease

TLS Online TPP Program

#Question id: 12923

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Name the technology which is used as a means of identifying the initial concentrations of DNA or cDNA template used.

TLS Online TPP Program

#Question id: 12924

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

In order to quantify unknown DNA templates, a standard dilution is prepared using DNA of known concentration. As the DNA accumulates during the early exponential phase of the reaction,an arbitrary point is taken where each of the diluted DNA samples cross. This is termed as_____

TLS Online TPP Program

#Question id: 12925

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Another name of TaqMan assay is ____