In a two-stage chemostat system, the volumes of the first and second reactors are V₁ = 500 l and V₂ = 300 l, respectively. The first reactor is used for biomass production and the second is for a secondary metabolite formation. The feed flow rate to the first reactor is F = 100 l/h, and the glucose concentration in the feed is S = 5.0 g/l. Use the following constants for the cells.

Determine cell concentration in the effluent of the first stage.

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#Question id: 18647

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following technique used discontinuous type gel system

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#Question id: 18663

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

When a protein exists in mono-, di- and tri-phosphorylated forms. The difference of a couple of phosphate groups has no significant effect on the overall relative molecular mass of the protein, hence a single band on SDS gels, but the small charge difference introduced on each molecule can be detected by

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#Question id: 18668

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Enzymes and antibody can easily be detected in samples subjected to electrophoresis on cellulose acetate, by using the 

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#Question id: 18672

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Larger DNA fragments up to 2 × 10 ³ kb can be separated by using

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#Question id: 18683

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Eukaryotic mRNA can be separated by using 

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#Question id: 18685

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the items in Group 1 with an appropriate description in Group 2 

Group I	Group 2
P. UPGMA	1. Protein sequence database
Q. CLUSTALW	2. Phylogenetic Analysis
R. SWISS-PROT	3. 3-D structure visualization
S. RasMol	4. Multiple sequence alignment