Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V₀ = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S₀ = 300 g/l and X₀ = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h^-1 , K_S = 0.5 g/l, and Y_X/S = 0.3 g dw/g glucose. Determine the concentration of glucose at t = 10 h at quasi-steady state

#Question id: 14228

#Section 5: Bioprocess Engineering and Process Biotechnology

A fermenter is maintained at 35⁰C by water circulating at a rate of 0.5 kg s^-1 in a cooling coil inside the vessel. The inlet and outlet temperatures of the water are 8⁰C and 15⁰C respectively. The length of the cooling coil is increased by 50%. In order to maintain the same fermentation temperature, the rate of heat removal must be kept the same. Determine the new cooling-water flow rate and outlet temperature by carrying out the following calculations. The heat capacity of the cooling water can be taken as 4.18 kJ kg^- 1 o C^- 1. If the length of the coil is increased by 50⁰C the area available for heat transfer, A ', also increases by 50% so that A' = 1.5 A. The value of the overall heat-transfer coefficient is not expected to change very much. For the new coil, what is the value of UA’  ______________

#Question id: 14573

#Section 2: General Biology

#Question id: 10784

#Section 6: Plant, Animal and Microbial Biotechnology

The precursor of phenolic compound is___

#Question id: 14527

#Section 1: Engineering Mathematics

What is the value of 

#Question id: 1917

#Section 2: General Biology

Boon et al. showed that syngeneic transplantable tumors that mutate such that they express strong transplantation antigens are rejected. They called these variants: