TLS Online TPP Program

#Question id: 14195


Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. Determine the concentration of glucose at t = 10 h at quasi-steady state

#Section 5: Bioprocess Engineering and Process Biotechnology
  1. 0.52g/l  
  2. 0.167g/l
  3. 0.874g/l 
  4. 0.324g/l