Nutrient medium is to be heated from 10⁰C to 28⁰C is a single-pass countercurrent shell-and-tube heat exchanger before being pumped into a fed-batch fermenter. Medium passes through the tubes of the exchanger; the shell-side fluid is water which enters with flow rate 3 x 10^4 kg h^- 1 and temperature 60⁰C pre-heated medium is required at a rate of 50 m^3 h^-1. The density, viscosity and heat capacity of the medium are the same as water; the thermal conductivity of the medium is 0.54 Wm^- 1 ⁰C ^- 1. It is proposed to use 30 steel tubes with inner diameter 5 cm; the tubes will be arranged in line. The pipe wall is 5-mm thick; the thermal conductivity of the metal is 50 Wm^- 1 ⁰C^ - 1. The maximum linear shell-side fluid velocity is estimated as 0.15 m s^-1. What tube length is required? ____________

TLS Online TPP Program

#Question id: 12943

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Choose the correct order for the steps involve in Insitu hybridization.

1. Cell permeaabilisation

2. Detection of hybridised probe radioactive.

3. Wash excess probe from section

4. Cell fixation

TLS Online TPP Program

#Question id: 12944

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the following-

A.Tyrosine kinase.	1. Kaposis sacroma
B.HIV Transactivator.	2. Hypertension
C. Angiotensinogen.	3.Atherosclerosis
D.CET protein	4. Cardiac hypertrophy

TLS Online TPP Program

#Question id: 12945

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

It is a PCR based multiplex assay that allows a number of target sites such as deletions, duplications, mutations to be amplified with a pair of primer containing probes.

TLS Online TPP Program

#Question id: 12946

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Choose the incorrect statement for the Multiple Ligation dependent probe amplification.

TLS Online TPP Program

#Question id: 12947

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the following-

A. G- proteins.	1. src
B. Growth factors.	2. erbB
C. Protein kinases.	3. N-ras
D. Growth factor receptor.	4. hst

TLS Online TPP Program

#Question id: 12948

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Proteolytic cleavage of DNA polymerase by subtilisin caused loss of its nick translation activity. Which of the following properties is lost?