Ethanol is produced by anaerobic fermentation of glucose by Saccharomyces cerevisiae. For the particular strain of S. cerevisiae employed, the maintenance coefficient is 0.18 kg kg^{- 1} h^{- 1}, Yxs is 0.11 kg kg^-1, Y_PX is 3.9 kg kg^-1 and µ_max is 0.4 h^-1.  It is decided to investigate the possibility of using Zymomonas mobilis bacteria instead of yeast for making ethanol. Z. mobilis is known to produce ethanol under anaerobic conditions using a different metabolic pathway to that employed by yeast. Typical values of Yxs are lower than for yeast at about 0.06 kg kg^-1; on the other hand, the maintenance coefficient is higher at 2.2 kg kg ^-1 h ^-1. Ypx for Z. mobilis is 7.7 kg kg^-l; µ_max is 0.3 h- 1.  From stoichiometry, what is the maximum theoretical yield of ethanol from glucose                 

TLS Online TPP Program

#Question id: 12939

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Choose the incorrect statement for the production of site-directed mutations.

TLS Online TPP Program

#Question id: 12940

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

 Choose the correct order for the steps involve in Protein engineering.

1. Protein modelling

2. Protein crystallography

3. Site-directed mutations

4. Comparison with entries in databases of known proteins.

TLS Online TPP Program

#Question id: 12941

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Name the therapeutic protein which has been used to successfully treat certain types of breast cancer.

TLS Online TPP Program

#Question id: 12942

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Name a single - stranded uracil containing vector which is grown in a selectable host deficit of the enzymes.

TLS Online TPP Program

#Question id: 12943

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Choose the correct order for the steps involve in Insitu hybridization.

1. Cell permeaabilisation

2. Detection of hybridised probe radioactive.

3. Wash excess probe from section

4. Cell fixation

TLS Online TPP Program

#Question id: 12944

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the following-

A.Tyrosine kinase.             1. Kaposis sacroma

B.HIV Transactivator.      2. Hypertension

C. Angiotensinogen.         3.Atherosclerosis

D.CET protein                      4. Cardiac hypertrophy