A steam steriliser is used to sterilise liquid medium for fermentation. The initial concentration of contaminating organisms is 10⁸ per litre. For design purposes, the final acceptable level of contamination is usually taken to be 10^-3cells; this corresponds to a risk that one batch in a thousand will remain contaminated even after the sterilisation process is complete. For how long should 1 m³ medium be treated if the temperature is 121⁰C ________________.

 To be safe, assume that the contaminants present are spores of Bacillus stearothermophilus, one of the most heat-resistant microorganisms known. For these spores the activation energy for thermal death is 283 kJ gmo1^-1 and the Arrhenius constant is 10^36.2 s^-1

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#Question id: 14394

#Section 5: Bioprocess Engineering and Process Biotechnology

1.0 timol of an enzyme that contains five active sites per molecule converts 1.0 pmol substrate every 2 microseconds. Calculate catalytic centre activity (sec^-1). ......10^5

TLS Online TPP Program

#Question id: 14395

#Section 5: Bioprocess Engineering and Process Biotechnology

An enzyme is available at a specific activity of 500 units/mg protein. For an assay, 10miro mole substrate need to be converted per minute. What mass (in mg) of protein would you take for this to be achieved? 

TLS Online TPP Program

#Question id: 14396

#Section 5: Bioprocess Engineering and Process Biotechnology

A purified hydrolytic enzyme (Mr 150 000) is available with the description: Gives a Vmax of 3 200 units/mg protein under optimal conditions. How many micromoles of substrate can be hydrolysed by 1 mg of protein in 1 minute  

TLS Online TPP Program

#Question id: 14397

#Section 5: Bioprocess Engineering and Process Biotechnology

A purified hydrolytic enzyme (Mr 150 000) is available with the description: Gives a Vmax of 3 200 units/mg protein under optimal conditions. How many nmol of enzyme are there in 1 mg  

TLS Online TPP Program

#Question id: 14398

#Section 5: Bioprocess Engineering and Process Biotechnology

The internal effectiveness factor is useful because , which statement is not correct 

TLS Online TPP Program

#Question id: 14399

#Section 5: Bioprocess Engineering and Process Biotechnology

 observable Thiele modulus CD decreases when