Aspartase enzyme is used industrially for manufacture of aspartic acid, a component of low-calorie sweetener. Fumaric acid and ammonia are converted to aspartic acid according to the equation:

 

                                        

Under investigation is a process using aspartase in intact Bacillus cadaveris cells. In the substrate range of interest, the conversion can be described using Michaelis-Menten kinetics with K m 4.0 gl^-1. The substrate solution contains 15% (w/v) ammonium fumarate; enzyme is added in the form of lyophilised cells and the reaction stopped when 85% of the substrate is converted. At 32⁰C vmax for the enzyme is 5.9 g l^-1h^-1 and its half-life is 10.5 d. At 37⁰C Vmax increases to 8.5 g l^{- 1} h^{- I} but the half-life is reduced to 2.3 d. The average downtime between batch reactions is 28 h. calculate the reactor volume required to produce 5000 tonnes of aspartic acid per year

#Question id: 10807

#Section 6: Plant, Animal and Microbial Biotechnology

Cyanogenic Glycosides and Glucosinolates are nitrogen containing secondary metabolites in plants. Following are some statements regarding the action of Cyanogenic Glycosides and Glucosinolates;

a) Cyanogenic Glycosides release the poison hydrogen cyanide by the action of enzyme known as Hydroxynitrile lyase

b) Release of the mustard-smelling volatiles from glucosinolates is catalyzed by a hydrolytic enzyme, called a thioglucosidase or myrosinase

c) substerate for Glycosidase is Cyanogenic glycoside and Thioglucosidase is Aglycone

d) Like cyanogenic glycosides, glucosinolates are stored in the intact plant separately from the enzymes that hydrolyze them, and they are brought into contact with these enzymes only when the plant is crushed

Which of the following combinations of above statement is true?

#Question id: 10808

#Section 6: Plant, Animal and Microbial Biotechnology

Many plants also contain unusual amino acids, called nonprotein amino acids, these Nonprotein amino acids are often very similar to common protein amino acids. Canavanine, for example, is a close analog of_____A______, and azetidine-2-carboxylic acid has a structure very much like that of_____B________.

#Question id: 11050

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Three dimensional reconstructions of Arabidopsis sieve plates using a staining technique that can be used to image entire plant organs and Open sieve pores are visible as well as callose plug are also visible which technique used to analyze

#Question id: 11344

#Section 3: Genetics, Cellular and Molecular Biology

Inner membrane is rich in which type of phospholipids?

#Question id: 11345

#Section 3: Genetics, Cellular and Molecular Biology

Which enzyme of citric acid cycle present in inner membrane of mitochondria?

#Question id: 11347

#Section 3: Genetics, Cellular and Molecular Biology

Which one of the following enzymes is NOT a part of pyruvate dehydrogenase enzyme complex in glycolysis pathway?