Aspartase enzyme is used industrially for manufacture of aspartic acid, a component of low-calorie sweetener. Fumaric acid and ammonia are converted to aspartic acid according to the equation:

 

                                        

Under investigation is a process using aspartase in intact Bacillus cadaveris cells. In the substrate range of interest, the conversion can be described using Michaelis-Menten kinetics with K m 4.0 gl^-1. The substrate solution contains 15% (w/v) ammonium fumarate; enzyme is added in the form of lyophilised cells and the reaction stopped when 85% of the substrate is converted. At 32⁰C vmax for the enzyme is 5.9 g l^-1h^-1 and its half-life is 10.5 d. At 37⁰C Vmax increases to 8.5 g l^{- 1} h^{- I} but the half-life is reduced to 2.3 d. The average downtime between batch reactions is 28 h. calculate the reactor volume required to produce 5000 tonnes of aspartic acid per year

#Question id: 3216

#Section 3: Genetics, Cellular and Molecular Biology

The frequency of galactosemia is approximately 1 in 40,000 live births. The frequency of the carrier state can be calculated as

#Question id: 3250

#Section 3: Genetics, Cellular and Molecular Biology

Consider a diploid population at Hardy-Weinberg equilibrium. For a locus with two alleles, the frequency of the A1A1, A1A2 and A2A2 genotype is 0.01, 0.18 and 0.81) Which of the following statement is correct?

#Question id: 3251

#Section 3: Genetics, Cellular and Molecular Biology

Looking at 3 allele per locus and following Hardy Weinberg equilibrium what will be the expected heterozygosity if q1=0.5, q2=0.3, q3=0.2

#Question id: 3252

#Section 3: Genetics, Cellular and Molecular Biology

 The unaffected brother of a cystic fibrosis affected girl is referred for genetic counseling. Assume that the population is in Hardy-Weinberg equilibrium. Which of the following is correct?  (Cystic fibrosis is an autosomes recessive inheritance with an incidence of 1 in 2500).

#Question id: 3253

#Section 3: Genetics, Cellular and Molecular Biology

In a population 64% individuals of 1000 population are affected with autosomes dominant diseased. How many individuals are expected carry at least one dominant allele?

#Question id: 3254

#Section 3: Genetics, Cellular and Molecular Biology

Consider a single locus with 2 alleles which are at Hardy-Weinberg equilibrium. If the frequency of the heterozygous genotypes is 0.42, what is the frequency of one homozygote in the population?