Aspartase enzyme is used industrially for manufacture of aspartic acid, a component of low-calorie sweetener. Fumaric acid and ammonia are converted to aspartic acid according to the equation:

 

                                        

Under investigation is a process using aspartase in intact Bacillus cadaveris cells. In the substrate range of interest, the conversion can be described using Michaelis-Menten kinetics with K m 4.0 gl^-1. The substrate solution contains 15% (w/v) ammonium fumarate; enzyme is added in the form of lyophilised cells and the reaction stopped when 85% of the substrate is converted. At 32⁰C vmax for the enzyme is 5.9 g l^-1h^-1 and its half-life is 10.5 d. At 37⁰C Vmax increases to 8.5 g l^{- 1} h^{- I} but the half-life is reduced to 2.3 d. The average downtime between batch reactions is 28 h. calculate the reactor volume required to produce 5000 tonnes of aspartic acid per year

TLS Online TPP Program

#Question id: 14413

#Section 2: General Biology

Which of the following cannot be used to sterilize a heat-labile solution stored in a plastic container?

TLS Online TPP Program

#Question id: 14414

#Section 2: General Biology

How Aldehydes work as effective antimicrobial agent?

TLS Online TPP Program

#Question id: 14415

#Section 2: General Biology

Which of the following physical sterilization processes can denature proteins?

1. Boiling

2. Autoclaving

3. Pasteurization

4. Refrigeration

5. Ionizing

Choose the incorrect combination.

TLS Online TPP Program

#Question id: 14416

#Section 2: General Biology

Bacteriostatic and Bactericidal functions can be achieved by

TLS Online TPP Program

#Question id: 14417

#Section 5: Bioprocess Engineering and Process Biotechnology

Which size is suitable for following, match the following?

Products	Filter Pore Diameter (nm)
I. Yeast and fungi	p. 0.6–1.2
II. Bacteria	q. 0.4–0.8
III. Antibiotics	r. 300–103
IV. Organic acid	s. 103–104

TLS Online TPP Program

#Question id: 14418

#Section 5: Bioprocess Engineering and Process Biotechnology

It is required to provide a 20-m^3 fermenter with air at a rate of 10 m^3 min ^-I for a fermentation lasting 100 hours. From an investigation of the filter material to be used, the optimum linear air velocity was shown to be 0.15 m sec^-I, at which the value of K was 1.535 cm^-I. calculate The dimensions (radius and depth) of the filter? The air in the fermentation plant contained approximately 200 micro-organisms m^-3. The acceptable degree of contamination is one in a thousand.

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