Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  Determine the volume of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics. 

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#Question id: 2142

#Section 2: General Biology

Glucose transport into erythrocytes is an example of

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#Question id: 154

#Section 2: General Biology

The peptide bond is which of the following?

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#Question id: 16436

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The PCR consists of three defined sets of times and temperatures, termed steps

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#Question id: 2950

#Section 3: Genetics, Cellular and Molecular Biology

During which phases of the cell cycle do the chromosomes exist in a doubled state?

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#Question id: 347

#Section 4: Fundamentals of Biological Engineering

A beaker contains 100 milliliters (mL) of NaOH solution at pH = 13. A technician carefully pours into the beaker 10 mL of HCl at pH = 1. Which of the following statements correctly describes the result of this mixing?