Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  Determine the volume of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics. 

TLS Online TPP Program

#Question id: 18683

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Eukaryotic mRNA can be separated by using 

TLS Online TPP Program

#Question id: 18685

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the items in Group 1 with an appropriate description in Group 2 

Group I	Group 2
P. UPGMA	1. Protein sequence database
Q. CLUSTALW	2. Phylogenetic Analysis
R. SWISS-PROT	3. 3-D structure visualization
S. RasMol	4. Multiple sequence alignment

TLS Online TPP Program

#Question id: 18686

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Homology modeling may be distinguished from ab initio prediction because: 

TLS Online TPP Program

#Question id: 18687

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Homology modeling involves 

TLS Online TPP Program

#Question id: 18688

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the items in Group 1 with an appropriate description in Group 2 

Group I	Group 2
P. GOR method	1. Protein structure visualization
Q. Genome sequencing	2. Sanger method
R. ESTs	3. Protein secondary structure prediction
S. RasMol	4. gene expressed as cDNA

TLS Online TPP Program

#Question id: 18689

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Ab initio approach makes structural predictions based on