Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  If Yp/s is 4 mg Mab/g glu, determine productivity of the system? __________________

#Question id: 1129

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following plays a role in the relaxation of smooth muscle cells by nitric oxide?

a. cGMP          b. protein kinase A      c. protein kinase G      d. guanylyl cyclise

#Question id: 1130

#Section 3: Genetics, Cellular and Molecular Biology

In muscle cells, glycogen phosphorylase kinase is activated by

a. Ca2+.           b. diacylglycerol.   c. nitric oxide.       d. protein kinase A.    

#Question id: 1133

#Section 3: Genetics, Cellular and Molecular Biology

What tertiary structure characterizes all cytokines?

#Question id: 1134

#Section 3: Genetics, Cellular and Molecular Biology

Arrange the following proteins in the proper order in which they become activated in the MAPK signaling pathway:

a. MAP kinase                         b. MEK           c. Ras              d. Raf              e. RTK            

#Question id: 1136

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following signaling pathways can be activated by activation of receptor tyrosine kinases?

a. phospholipase Cγ                b. PI-3 kinase

c. MAP kinase                         d. none of the above

#Question id: 1137

#Section 3: Genetics, Cellular and Molecular Biology

A loss-of-function mutation in PTEN phosphatase results in

a. increased cell proliferation.            

b. decreased cell proliferation.

c. increased apoptosis.                                   

d. decreased apoptosis.