C. roseus cells immobilized in Ca-alginate beads of diameter 0.5 mm are used for production of indole alkaloids (IA) in a fluidized-bed bioreactor. The rate limiting nutrient is glucose and no intraparticle diffusion limitations exist. Use the following data: Flow rate of the feed: Q = 1 l/h, Glucose in the feed: So = 30 g/l, Plant Cell Concentration: X = 6 g/l reac. The rate constant for IA formation: k = 5 d-1 (g/l)^-1 Ks = 0.4 g/l, Column diameter: Do = 0.15 m. Growth is negligible and Monod kinetics is valid. If Yp/s is 0.02 g IA/g glu, determine IA concentration in the effluent.

_______________

TLS Online TPP Program

#Question id: 27169

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the option is not suitable for given alignment

TLS Online TPP Program

#Question id: 27170

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Pfam database contains information of

TLS Online TPP Program

#Question id: 27171

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The percentage SIMILARITIES and IDENTITIES, respectively, between the two peptide sequences given below will be ______% and _____%.

Peptide I :

Phe-Ala-Arg-Arg-Gln-Trp-Leu-Thr-Phe-Thr

Peptide II:

Tyr-Ala-Lys-Glu-Gln-Tyr-Ile-Ser-Phe-Thr

TLS Online TPP Program

#Question id: 27172

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which option is correct for EMBL format?

TLS Online TPP Program

#Question id: 27175

#Section 5: Bioprocess Engineering and Process Biotechnology

A bacterial culture having a specific oxygen uptake rate of 5 mmol O2 (g- DCW)^-1h-1 is being grown aerobically in a fed-batch reactor. The maximum value of the volumetric oxygen transfer coefficient is 0.18 s for the stirred tank bioreactor and the critical dissolved oxygen concentration is 20% of the saturation concentration (8 mg L^-1). The maximum density to which the cells can be grown in the fed-batch process, without the growth being limited by oxygen transfer, is approximately

TLS Online TPP Program

#Question id: 27176

#Section 5: Bioprocess Engineering and Process Biotechnology

A strain of Azotobacter vinelandii is cultured in a 15-m³ stirred fermenter for alginate production. Under current operating conditions, ka is 0.17 s¹. The solubility of oxygen in the broth is approximately 8 × 10^-3 kg m ^-3.

(a) The specific rate of oxygen uptake is 12.5 mmol g^-1h^-1. What is the maximum cell concentration supported by oxygen transfer in the fermenter.............in g/l