C. roseus cells immobilized in Ca-alginate beads of diameter 0.5 mm are used for production of indole alkaloids (IA) in a fluidized-bed bioreactor. The rate limiting nutrient is glucose and no intraparticle diffusion limitations exist. Use the following data: Flow rate of the feed: Q = 1 l/h, Glucose in the feed: So = 30 g/l, Plant Cell Concentration: X = 6 g/l reac. The rate constant for IA formation: k = 5 d-1 (g/l)^-1 Ks = 0.4 g/l, Column diameter: Do = 0.15 m. Growth is negligible and Monod kinetics is valid. If Yp/s is 0.02 g IA/g glu, determine IA concentration in the effluent.

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#Question id: 379

#Section 4: Fundamentals of Biological Engineering

A compound has a pKa of 7.4. To 100 mL of a 1.0 M solution of this compound at pH 8.0 is added 30 mL of 1.0 M hydrochloric acid. The resulting solution is pH:

#Question id: 380

#Section 4: Fundamentals of Biological Engineering

The Henderson-Hasselbalch equation:

#Question id: 381

#Section 4: Fundamentals of Biological Engineering

Consider an acetate buffer, initially at the same pH as its pKa (4.76). When sodium hydroxide (NaOH) is mixed with this buffer, the:

#Question id: 382

#Section 4: Fundamentals of Biological Engineering

The molar ratio of sodium acetate to acetic acid in a buffer solution with a pH OF 5.76 is 10:1. Assuming the total buffer concentration is 2.2 X 10^-2 mol/L, how many grams of sodium acetate (m.w. 82) and how many grams of acetic acid (m.w. 60) should be used in preparing a litre of the solution?

#Question id: 383

#Section 4: Fundamentals of Biological Engineering

Three buffers are made by combining a 1 M solution of acetic acid with a 1 M solution of sodium acetate in the ratios shown below

 Which of these statements is true of the resulting buffers?

#Question id: 384

#Section 4: Fundamentals of Biological Engineering

A solution was prepared by dissolving 0.02 moles of acetic acid (HOAc; pKa = 4.8) in water to give 1 liter of solution. What is the pH? To this solution was then added 0.008 moles of concentrated sodium hydroxide (NaOH). What is the new pH? (In this problem, you may ignore changes in volume due to the addition of NaOH).