Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. Determine the concentration of cells at quasi-steady state when t = 10 h.

#Question id: 14158

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 14159

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 14160

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 14161

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 14162

#Section 4: Fundamentals of Biological Engineering

A continuous process is set up for treatment of wastewater. Each day, 10⁵ kg cellulose and 10³ kg bacteria enter in the feed stream, while 10⁴ kg cellulose and 1.5 x 10⁴ kg bacteria leave in the effluent. The rate of cellulose digestion by the bacteria is 7 x 10⁴ kg d^-1. The rate of bacterial growth is 2 x 10⁴ kg d^-1 ; the rate of cell death by lysis is 5 x 10² kg d^-1. Write balances for cellulose and bacteria in the system.

#Question id: 14162

#Section 5: Bioprocess Engineering and Process Biotechnology

A continuous process is set up for treatment of wastewater. Each day, 10⁵ kg cellulose and 10³ kg bacteria enter in the feed stream, while 10⁴ kg cellulose and 1.5 x 10⁴ kg bacteria leave in the effluent. The rate of cellulose digestion by the bacteria is 7 x 10⁴ kg d^-1. The rate of bacterial growth is 2 x 10⁴ kg d^-1 ; the rate of cell death by lysis is 5 x 10² kg d^-1. Write balances for cellulose and bacteria in the system.