Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. Determine the concentration of cells at quasi-steady state when t = 10 h.

TLS Online TPP Program

#Question id: 15475

#Section 4: Fundamentals of Biological Engineering

Production of single-cell protein from hexadecane is described by the following reaction equation:

C₁₆H₃₄ + aO₂ + bNH₃ cCH_l.66O_0.27N0_.20 + dCO ₂ + eH₂O

where CH _1.660_0.27N_0.20 represents the biomass. If RQ= 0.43, determine the stoichiometric coefficients.

TLS Online TPP Program

#Question id: 15476

#Section 6: Plant, Animal and Microbial Biotechnology

Industrial production of amino acids are done because they are used extensively in the 

1. flavor enhancers

2. Fabric

3. nutritional supplements

4. feed additives

5. medicine 

Choose the most appropriate option

TLS Online TPP Program

#Question id: 15477

#Section 6: Plant, Animal and Microbial Biotechnology

Why these metabolites are manufactured by microbial fermentation rather than chemical synthesis 

TLS Online TPP Program

#Question id: 15478

#Section 6: Plant, Animal and Microbial Biotechnology

Escherichia coli preferentially utilizes glucose by preventing the uptake of lactose and switching off transcription of the lac operon. This mechanism is known as 

TLS Online TPP Program

#Question id: 15479

#Section 6: Plant, Animal and Microbial Biotechnology

Out of the following what usually supports the fastest growth rate and is therefore considered the preferred nitrogen source for E. coli

TLS Online TPP Program

#Question id: 15480

#Section 6: Plant, Animal and Microbial Biotechnology

Feedback repression involves the turning “off” of enzyme synthesis when the amount of the product has been made in sufficient quantities. The end product of the pathway acts as