Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. If qP = 0.05 g product/g cells h and P₀ = 0.1 g/l, determine the product concentration in the vessel at t = 10 h

TLS Online TPP Program

#Question id: 12915

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following statement is not correct about PCR.

TLS Online TPP Program

#Question id: 12916

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which statement is correct for Zoo blotting?

TLS Online TPP Program

#Question id: 12917

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Arrange the steps of stages in PCR in correct order.

1. The region to be specifically amplified is made accessible within the complex DNA.

2. The double stranded DNA is denatured by heating the reaction mixture to above 90 degree Celsius.

3. A thermal cycler is used to incrementally decrease the annealing temperature.

4. Reaction mixture is then cooled to a temperature between 40 and 60 degree celsius.

TLS Online TPP Program

#Question id: 12918

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Name the enzyme which helps in the DNA synthesis in the PCR.

TLS Online TPP Program

#Question id: 12919

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following statement is not correct about RT-PCR. 

TLS Online TPP Program

#Question id: 12920

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The development of isothermal systems such as the ____ DNA amplification system do away with the need for a thermal cycler and have the advantage of being able to be used outside the laboratory.