Pseudomonas methylotrophus is used to produce single-cell protein from methanol in a 1000 m³ pressure-cycle airlift fermenter. The biomass yield from substrate is 0.41 g g^{- 1}, K_S is 0.7 mg l^{- 1}, and the maximum specific growth rate is 0.44 h^{- 1}. The medium contains 4% (w/v) methanol. A substrate conversion of 98% is desirable. The reactor may be operated either in batch or continuous mode. If operated in batch, an inoculum of 0.01% (w/v) is used and the downtime between batches is 20 h. If operated continuously, a downtime of 25 d is expected per year. Neglecting maintenance requirements, compare the annual biomass production from batch and continuous reactors?

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TLS Online TPP Program

#Question id: 12921

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the following:-

A. Gene probe production.	i. Novel proteins
B. Genome mapping.	ii. PCR mutagenesis
C. Protein Engineering.	iii. Sequence tagged sites
D. Gene Editing.	iv. Use with blots

TLS Online TPP Program

#Question id: 12922

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

 Match the following:-

1. Ligase chain reaction.	i. Mutation detection
2. Nucleic acid sequence based amplification.	ii. Non isothermal
3. Loop mediated isothermal amplification.	iii. Viral detection
4. Branched DNA amplification.	iv. parasitic disease

TLS Online TPP Program

#Question id: 12923

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Name the technology which is used as a means of identifying the initial concentrations of DNA or cDNA template used.

TLS Online TPP Program

#Question id: 12924

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

In order to quantify unknown DNA templates, a standard dilution is prepared using DNA of known concentration. As the DNA accumulates during the early exponential phase of the reaction,an arbitrary point is taken where each of the diluted DNA samples cross. This is termed as_____

TLS Online TPP Program

#Question id: 12925

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Another name of TaqMan assay is ____

TLS Online TPP Program

#Question id: 12926

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

PCR is undertaken with a fluorescence reporter / quencher pair called______