Pseudomonas methylotrophus is used to produce single-cell protein from methanol in a 1000 m³ pressure-cycle airlift fermenter. The biomass yield from substrate is 0.41 g g^{- 1}, K_S is 0.7 mg l^{- 1}, and the maximum specific growth rate is 0.44 h^{- 1}. The medium contains 4% (w/v) methanol. A substrate conversion of 98% is desirable. The reactor may be operated either in batch or continuous mode. If operated in batch, an inoculum of 0.01% (w/v) is used and the downtime between batches is 20 h. If operated continuously, a downtime of 25 d is expected per year. Neglecting maintenance requirements, compare the annual biomass production from batch and continuous reactors?

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TLS Online TPP Program

#Question id: 19916

#Section 5: Bioprocess Engineering and Process Biotechnology

Which of the following is used to calculate mass of substrate in the reactor?

TLS Online TPP Program

#Question id: 19927

#Section 5: Bioprocess Engineering and Process Biotechnology

Fluidized bed bioreactors provide higher mass transfer rates than packed bed bioreactors because

TLS Online TPP Program

#Question id: 19928

#Section 5: Bioprocess Engineering and Process Biotechnology

A chemostat has a liquid volume of 2 litres and is being fed at a rate of 4 litres per hour. Dilution rate for this reactor will be

TLS Online TPP Program

#Question id: 19929

#Section 5: Bioprocess Engineering and Process Biotechnology

he growth of an organism on glucose is described by the following Monod model parameters: μm = 0.5 h^-1 and Ks =0.1 g.l^-1, if the concentration of glucose in the feed is 10 g.l^-1 and the dilution rate is set to 0.4 h^-1, then the steady state concentration of glucose in the effluent will be

TLS Online TPP Program

#Question id: 19954

#Section 5: Bioprocess Engineering and Process Biotechnology

A continuous reactor at steady state contains 0.04 g.l^-1 of biomass and 0.02 g.l^-1 of phenol. The feed contained 0.1 g.l^-1 of phenol. The biomass yield would be

TLS Online TPP Program

#Question id: 19955

#Section 5: Bioprocess Engineering and Process Biotechnology

A culture system with constant environmental conditions maintained through continual provision of nutrient and removal of wastes is called __________ culture system.