A two-stage chemostat system is used for production of secondary metabolite. The volume of each reactor is 0.5 m³; the flow rate of feed is 50 l h^{- 1}. Mycelial growth occurs in the first reactor; the second reactor is used for product synthesis. The concentration of substrate in the feed is 10 gl^-1. Kinetic and yield parameters for the organism are:

                                                            

Assume that product synthesis is negligible in the first reactor and growth is negligible in the second reactor. What is the overall substrate conversion?

_______________

TLS Online TPP Program

#Question id: 14930

#Section 5: Bioprocess Engineering and Process Biotechnology

E. coli grows from 0.10 to 0.50 kg dry cell m^-3 in 1 h. Assuming exponential growth during this period, evaluate the specific growth rate 

TLS Online TPP Program

#Question id: 14931

#Section 5: Bioprocess Engineering and Process Biotechnology

E. coli grows from 0.10 to 0.50 kg dry cell m^-3 in 1 h. Evaluate the doubling time during the exponential growth phase 

TLS Online TPP Program

#Question id: 14932

#Section 5: Bioprocess Engineering and Process Biotechnology

 E. coli grows from 0.10 to 0.50 kg dry cell m^-3 in 1 h. How much time would be required to grow from 0.10 to 1.0 kg dry cell m^-3? You may assume exponential growth during this period 

TLS Online TPP Program

#Question id: 14933

#Section 5: Bioprocess Engineering and Process Biotechnology

E. coli was continuously cultured in a continuous stirred tank fermenter with a working volume of 1.0 I by chemostat. A medium containing 4.0 g1^-1 of glucose as a carbon source was fed to the fermenter at a constant flow rate of 0.5 I h^-1, and the glucose concentration in the output stream was 0.20 g1^-1, The cell yield with respect to glucose YxS was 0.42 g dry cells per gram glucose. Determine the cell concentration in the output stream 

TLS Online TPP Program

#Question id: 14934

#Section 5: Bioprocess Engineering and Process Biotechnology

E. coli was continuously cultured in a continuous stirred tank fermenter with a working volume of 1.0 I by chemostat. A medium containing 4.0 g1^-1 of glucose as a carbon source was fed to the fermenter at a constant flow rate of 0.5 I h^-1, and the glucose concentration in the output stream was 0.20 g I^-1. The cell yield with respect to glucose YxS was 0.42 g dry cells per gram glucose. Determine specific growth rate 

TLS Online TPP Program

#Question id: 14935

#Section 1: Engineering Mathematics