Cellulomonas bacteria used as single-cell protein for human or animal food are produced from glucose under anaerobic conditions. All carbon in the substrate is converted into biomass; ammonia is used as nitrogen source. The molecular formula for the biomass is CH_I.560_0.54N_0.16; the cells also contain 5% ash. How does the yield of biomass from substrate in mass and molar terms compare with the maximum possible biomass yield?

#Question id: 2108

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following is TRUE regarding lipid-anchored membrane proteins?

#Question id: 19719

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 14166

#Section 4: Fundamentals of Biological Engineering

Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is:

You have been put in charge of a small fermenter for growing the bacteria and aim to produce 2.5 kg of cells for inoculation of a pilot-scale reactor. What minimum amount of hexadecane substrate must be contained in your culture medium? 

#Question id: 363

#Section 4: Fundamentals of Biological Engineering

What is the pH of a mixture of 0.042 M NaH2PO4 and 0.058 M Na2HPO4? If 1.0 mL of 10.0 M NaOH is added to a liter of the buffer prepared, how much will the pH change? (pKa = 6.86)

#Question id: 373

#Section 4: Fundamentals of Biological Engineering

What is the hydroxyl ion (OH-) concentration of a solution of pH 8?