TLS Online TPP Program

#Question id: 14319


Both Saccharomyces cerevisiae yeast and Zymomonas mobilis bacteria produce ethanol from glucose under anaerobic conditions without external electron acceptors. The biomass yield from glucose is 0.11 g g-1 for yeast and 0.05 g g-I for Z. mobilis. In both cases the nitrogen source is NH3. Both cell compositions are represented by the formula CH1.800.5N0.2. What is the yield of ethanol from glucose by Saccharomyces cerevisiae yeast?

#Section 5: Bioprocess Engineering and Process Biotechnology
  1. 1.72gmol gmol-1
  2. 1.82gmol gmol-1  
  3. 1.90gmol gmol-1 
  4. 1.50gmol gmol-1                                    
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TLS Online TPP Program

#Question id: 27558

#Section 5: Bioprocess Engineering and Process Biotechnology

10 kg of a pure enzyme (Mr 30 000) catalyses the conversion of 1.5 g of a substrate (Mr 44) to products in 5 minutes. What will be the turnover number in (sec^-1)  ....10^5

TLS Online TPP Program

#Question id: 27562

#Section 5: Bioprocess Engineering and Process Biotechnology

Match each parameter in Group 1 with the appropriate measuring device in Group 2

Group 1                                       Group 2

(P) Pressure                               (1) Photometer

(Q) Foam                                    (2) Rotameter

(R) Turbidity                               (3) Diaphragm gauge

S) Flow rate                                (4) Rubber sheathed electrode

TLS Online TPP Program

#Question id: 27563

#Section 5: Bioprocess Engineering and Process Biotechnology

Match the instruments in Group 1 with their corresponding measurements in Group 2
Group 1                                                         Group 2
(P) Manometer                                              (1) Agitator speed
(Q) Rotameter                                               (2) Pressure difference
(R) Tachometer                                              (3) Cell number
(S) Haemocytometer                                     (4) Air flow rate

TLS Online TPP Program

#Question id: 27589

#Section 5: Bioprocess Engineering and Process Biotechnology

The pressure gauge on an autoclave reads 15 psi. What is the absolute pressure in the chamber in psi? (Assume atmospheric pressure is 14.7 psi)

TLS Online TPP Program

#Question id: 27590

#Section 5: Bioprocess Engineering and Process Biotechnology

The pressure gauge on an autoclave reads 15 psi. What is the absolute pressure in the chamber in atm? (Assume atmospheric pressure is 14.7 psi and 1 psi=6.8*10-2 atm

TLS Online TPP Program

#Question id: 27591

#Section 5: Bioprocess Engineering and Process Biotechnology

Gas leaving a fermenter at close to 1 atm pressure and 25°C has the following composition: 78.2% nitrogen, 19.2% oxygen, 2.6% carbon dioxide. Calculate the mass composition of the fermenter off-gas.