Production of recombinant protein by a geneticallyengineered strain of Escherichia coli is proportional to cell growth. Ammonia is used as nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula CHI.5500.31N0.25. The yield of biomass from glucose is measured at 0.48 g g^-1; the yield of recombinant protein from glucose is about 20% that for cells. If the biomass yield remains at 0.48 g g^{- l}, how much change in oxygen requirement for wild-type E. coli unable to synthesise recombinant protein?

#Question id: 380

#Section 4: Fundamentals of Biological Engineering

The Henderson-Hasselbalch equation:

#Question id: 381

#Section 4: Fundamentals of Biological Engineering

Consider an acetate buffer, initially at the same pH as its pKa (4.76). When sodium hydroxide (NaOH) is mixed with this buffer, the:

#Question id: 382

#Section 4: Fundamentals of Biological Engineering

The molar ratio of sodium acetate to acetic acid in a buffer solution with a pH OF 5.76 is 10:1. Assuming the total buffer concentration is 2.2 X 10^-2 mol/L, how many grams of sodium acetate (m.w. 82) and how many grams of acetic acid (m.w. 60) should be used in preparing a litre of the solution?

#Question id: 383

#Section 4: Fundamentals of Biological Engineering

Three buffers are made by combining a 1 M solution of acetic acid with a 1 M solution of sodium acetate in the ratios shown below

 Which of these statements is true of the resulting buffers?

#Question id: 384

#Section 4: Fundamentals of Biological Engineering

A solution was prepared by dissolving 0.02 moles of acetic acid (HOAc; pKa = 4.8) in water to give 1 liter of solution. What is the pH? To this solution was then added 0.008 moles of concentrated sodium hydroxide (NaOH). What is the new pH? (In this problem, you may ignore changes in volume due to the addition of NaOH).

#Question id: 385

#Section 4: Fundamentals of Biological Engineering

In which reaction below does water not participate as a reactant (rather than as a product)?