Production of recombinant protein by a geneticallyengineered strain of Escherichia coli is proportional to cell growth. Ammonia is used as nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula CHI.5500.31N0.25. The yield ofbiomass from glucose is measured at 0.48 g g-1; the yield of recombinant protein from glucose is about 20% that for cells. If the biomass yield remains at 0.48 g g- l, how much change in ammonia requirement for wild-type E. coliunable to synthesise recombinant protein?

TLS Online TPP Program

#Question id: 13033

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The sample is then fractionated by electrophoresis through a _______. As all the proteins now have an _______ to mass ratio, they are separated on the basis of their _____.

TLS Online TPP Program

#Question id: 13034

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which the following statements is/are true about the stacking gel?

i. The stacking gel solution contains acrylamide.

ii. It is on the top of the separating gel.

iii. It is used to dilute the protein sample before it enters the main separating gel.

iv. It is into this gel that the wells are formed and the proteins loaded.

TLS Online TPP Program

#Question id: 13035

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Each band on the gel represents a different protein (or protein subunit); _____ proteins move through the gel more _____ than_____ proteins and therefore are found nearer the _____ of the gel.

TLS Online TPP Program

#Question id: 13036

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

In isoelectric focusing the protein will move until

TLS Online TPP Program

#Question id: 13037

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following proteins will be most far away from the positively charged electrode during isoelectric focusing in a buffer with pH of 5?

TLS Online TPP Program

#Question id: 13038

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Please arrange the following steps about ELIZA (Enzyme-linked immunosorbent assay) in chronological order

i. incubate with antibody-enzyme complex that binds primary antibody

ii. coat surface with antigen, block unoccupied sites with nonspecific protein

iii. add substrate, formation of colored product indicates presence of specific antigen

iv. incubate with primary antibody against specific antigen