Production of recombinant protein by a geneticallyengineered strain of Escherichia coli is proportional to cell growth. Ammonia is used as nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula CHI.5500.31N0.25. The yield ofbiomass from glucose is measured at 0.48 g g-1; the yield of recombinant protein from glucose is about 20% that for cells. If the biomass yield remains at 0.48 g g- l, how much change in ammonia requirement for wild-type E. coliunable to synthesise recombinant protein?

TLS Online TPP Program

#Question id: 27050

#Section 4: Fundamentals of Biological Engineering

In a process, two feed streams are there and one output stream. The feed streams have the feed rates as 500 kg/sec and 600 kg/sec. If the output stream rate is 800 kg/s, what is the mass stored in the chamber in five seconds?

TLS Online TPP Program

#Question id: 27051

#Section 4: Fundamentals of Biological Engineering

For the given drying process, a material (Called SRU) is dried and feed and outputs are as shown in the figure.

A, in kg is  

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#Question id: 27052

#Section 4: Fundamentals of Biological Engineering

A membrane is used for separation of gases from waste. If the feed stream contains 20% CO2 and 80% SO2 and the product contains 25% CO2 and 75% SO2. Assume that the waste stream amounts to 80% of the input stream. What is the composition of CO2 in waste stream?

TLS Online TPP Program

#Question id: 27053

#Section 4: Fundamentals of Biological Engineering

To obtain a 100 Kg of 40 % N2 solution, how much nitrogen must be added to a 20% nitrogen solution?

TLS Online TPP Program

#Question id: 27054

#Section 4: Fundamentals of Biological Engineering

For the given distillation column 1000 Kg of feed is given to the column and the efficiency of the column is 10%.  What is the amount of product?   

    

TLS Online TPP Program

#Question id: 27055

#Section 4: Fundamentals of Biological Engineering

What is the enthalpy of 150 g formic acid at 70°C and 1 atm relative to 25°C  and 1 atm? 

(Cp for formic acid is 0.524 cal g-1 °C-1)