In bacteria, some of the functions of eukaryotic cells are performed

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#Section 1: Engineering Mathematics

The maximum value of the determinant among all 2 x 2 real symmetric matrices with trace 24 is 

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#Section 2: General Biology

The simplest technique for isolating bacteria in growth media is referred to as the

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#Section 3: Genetics, Cellular and Molecular Biology

The excision (splicing) of many group I introns requires, in addition to the primary transcript RNA:

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#Section 2: General Biology

Eosinophils do NOT:

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#Question id: 13090

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 3’s plasmid is;