TLS Online TPP Program

#Question id: 3717


E. coli DNA polymerase catalyzes polymerization in the ________ direction(s)

#Section 3: Genetics, Cellular and Molecular Biology
  1. 5ʹ → 3ʹ

  2. 3ʹ → 5ʹ

  3. 2ʹ → 5ʹ

  4. both 5ʹ → 3ʹ and 3ʹ → 5ʹ

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TLS Online TPP Program

#Question id: 15493

#Section 4: Fundamentals of Biological Engineering

Assume that experimental measurements for a certain organism have shown that cells can convert two-thirds (wt/wt) of the substrate carbon to biomass carbon. Calculate the stoichiometric coefficients for the following biological reactions:

Glucose: C6H12O6 + aO2 + bNH3 c(C4.4H7.3N0.86O1.2) + dH2O + eCO2

TLS Online TPP Program

#Question id: 15494

#Section 4: Fundamentals of Biological Engineering

Assuming that cells convert two-thirds (w/w) of the substrate to biomass, the stoichiometric coefficient (1 only) for glucose utilisation for the following reaction is

TLS Online TPP Program

#Question id: 15495

#Section 4: Fundamentals of Biological Engineering

Calculate the degree of reduction for following substrates:
A) Methane
B) Glucose
C) Ethanol

TLS Online TPP Program

#Question id: 15496

#Section 4: Fundamentals of Biological Engineering

S. cerevisiae produces ethanol at a yield of 0.5 g/g glucose. The strain ferments 20g/l glucose in 24 hours. Calculate productivity of ethanol in this fermentation

TLS Online TPP Program

#Question id: 15497

#Section 4: Fundamentals of Biological Engineering

A. aceti converts alcohol to acetic acid according to the stoichiometric relation

C2H5OH + O2 🡪CH3COOH + H2O

In a vigorously agitated and aerated reactor containing 20 g/l ethanol, the organism produces 16 g/l acetic acid and 2 g/l was the residual ethanol concentration. What are the theoretical and observed yields of acetic acid expressed in g/g ethanol?

TLS Online TPP Program

#Question id: 15498

#Section 4: Fundamentals of Biological Engineering

The maximum yield for microbial conversion of Glucose (C6H12O6) to ethanol (C2H5OH) on a mol/mol basis is approximately: