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#Question id: 13062
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?
TLS Online TPP Program
#Question id: 3216
#Section 3: Genetics, Cellular and Molecular Biology
The frequency of galactosemia is approximately 1 in 40,000 live births. The frequency of the carrier state can be calculated as
TLS Online TPP Program
#Question id: 14610
#Section 2: General Biology
Which molecules are associated with the cell wall of Gram-positive bacteria?
1. Peptidoglycan
2. L-form amino acids
3. Teichoic acids
4. LPS
Which combination is correct?
TLS Online TPP Program
#Question id: 244
#Section 2: General Biology
The difference between dGMP and GMP is
TLS Online TPP Program
#Question id: 1576
#Section 2: General Biology
function of Properdin