TLS Online TPP Program

#Question id: 1091


Why does testosterone, a lipid-soluble signaling molecule that crosses the membranes of all cells, affect only target cells?

#Section 3: Genetics, Cellular and Molecular Biology
  1. Only target cells retain the appropriate genes regulated by testosterone.

  2. Intracellular receptors for testosterone are present only in target cells.

  3. Only target cells possess the cytosolic enzymes that transduce the signal from testosterone to adenylyl cyclase.

  4. Only in target cells is testosterone able to initiate the phosphorylation cascade leading to activated transcription factor.

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TLS Online TPP Program

#Question id: 18692

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which methods can be used for detection of self complementary regions of a sequence.

TLS Online TPP Program

#Question id: 14321

#Section 5: Bioprocess Engineering and Process Biotechnology

Both Saccharomyces cerevisiae yeast and Zymomonas mobilis bacteria produce ethanol from glucose under anaerobic conditions without external electron acceptors. The biomass yield from glucose is 0.11 g g-1 for yeast and 0.05 g g-I for Z. mobilis. In both cases the nitrogen source is NH 3. Both cell compositions are represented by the formula CH 1.800.5N0.2. Compare actual yield with theoretical maximum for Zymomonas mobilis bacteria ?

TLS Online TPP Program

#Question id: 13092

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
 
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
 
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
                              
Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

TLS Online TPP Program

#Question id: 253

#Section 2: General Biology

Which is not true of the different conformations of DNA?

TLS Online TPP Program

#Question id: 18686

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Homology modeling may be distinguished from ab initio prediction because: