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TLS Online TPP Program

#Question id: 149

The pKaʹs of the side chain group and the α-carboxyl group of glutamate are 4.1 and 2.1, respectively. Which statement accounts for this difference?

#Section 2: General Biology

  1. The side chain has more possible resonance structures.

  2. The α-carboxyl group has less steric hindrance and is therefore ionized more easily.

  3. The side chain is a different functional group than the α-carboxyl group.

  4. The α-carboxyl group is closer to the α-amino group than the side chain is.

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TLS Online TPP Program

#Question id: 12909

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

According to the diagram, arrange the steps in correct order: 


1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 
2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 
3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).
 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.
TLS Online TPP Program

#Question id: 12910

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the following:

A. RNA analysisi. Sexing or cell mutation sitesa. Expressed Sequence tag
B. Forensic scienceii. mRNA analysisb. Analysis of DNA from blood
C. Gene discoveryiii. DNA profilingc. Active latent viral infections
D. Single-cell analysisiv. RT–PCRd. Sex determination of unborn
TLS Online TPP Program

#Question id: 12911

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the following:

A. DNA polymerasesi. Alkaline phosphatasea. RNA-dependent DNA polymerase, used in cDNA synthesis           
B. Reverse transcriptaseii. T4 DNA ligaseb. Thermostable DNA polymerase used in PCR
C. Ligasesiii. AMV-RTc. Removes 5'-phosphates from DNA and RNA
D. Phosphatasesiv. Taq DNA pold. Links 5'-phosphate and 3'-hydroxyl ends via phosphodiester bond
TLS Online TPP Program

#Question id: 12912

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Name the enzyme which separates the double-helical DNA into single strands.
TLS Online TPP Program

#Question id: 12913

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

________  involves the size analysis of restriction fragments produced by several restriction enzymes individually and in combination. 
TLS Online TPP Program

#Question id: 12914

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

 In Electrophoresis of DNA the gel is first soaked in the alkali then it is transferred to the membrane so that the ________