What is the approximate charge difference between glutamic acid and alpha-ketoglutarate at pH 9.5?

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#Question id: 16436

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The PCR consists of three defined sets of times and temperatures, termed steps

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#Question id: 16438

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

^{A DNA sample having mass 1 picogram undergoes PCR, the eluted DNA is weighted about 1 microgram, the minimum number of cycles required}

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#Question id: 16440

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

In RNA isolation, for protein free solution what will be the ratio at  nm A260/A280

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#Question id: 16442

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Phenomenon of Smiling Effect is largely applicable in

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#Question id: 16444

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Gels of 15% polyacrylamide are useful for separating proteins in the range Mr = 100,000 to 10,000,  while a 7.5% gel are used to separate 

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#Question id: 16446

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Variants of PCR has been devised to enhance efficiency are