#Question id: 14238
#Section 5: Bioprocess Engineering and Process Biotechnology
A genetically-engineered strain of yeast is cultured in a bioreactor at 30⁰C for production of heterologous protein. The oxygen requirement is 80 mmol 1-1 h-I; the critical oxygen concentration is 0.004 mM. The solubility of oxygen in the fermentation broth is estimated to be 10% lower than in water due to solute effects. What mass-transfer coefficient is required if pure oxygen is used instead of air?
#Question id: 335
#Section 2: General Biology
Suppose the major solutes in intact lysosomes are KCl (~0.1 M) and NaCl (~0.03 M). When isolating lysosomes, what concentration of sucrose is required in the extracting solution at room temperature (25 °C) to prevent swelling and lysis?
#Question id: 10524
#Section 6: Plant, Animal and Microbial Biotechnology
Members of the Poaceae family, which includes all of the cereal crops, use benzoxazinoids, these alkaloids shows;
a) Alkaloids are derived from the tryptophan as constitutively produced defensive secondary metabolites
b) These benzoxazinoids such as DIBOA and its derivative DIMBOA are usually stored in the peroxisome
c) Upon damage, the inactive glycosides are hydrolyzed, generating glucosinolate, which are toxic not only to insect herbivores but also against pathogens
d) DIBOA and DIMBOA perform insecticidal and pesticidal activities
e) It can be inactivated by hydroxylation and N-glycosylation reactions
Which of the following combination is correct about benzoxazinoids alkaloids?
#Question id: 3714
#Section 3: Genetics, Cellular and Molecular Biology
Which DNA polymerase is most responsible for chain elongation in E. coli?
#Question id: 2952
#Section 3: Genetics, Cellular and Molecular Biology
Throughout interphase,.. ......... are synthesized at a relatively constant rate, whereas ............ are synthesized only during the S phase.