The hydrolysis of phosphoenolpyruvate proceeds with a DG'° of about –62 kJ/mol. The greatest contributing factors to this reaction are the destabilization of the reactants by electostatic repulsion and stabilization of the product pyruvate by:

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#Question id: 12942

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Name a single - stranded uracil containing vector which is grown in a selectable host deficit of the enzymes.

TLS Online TPP Program

#Question id: 12943

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Choose the correct order for the steps involve in Insitu hybridization.

1. Cell permeaabilisation

2. Detection of hybridised probe radioactive.

3. Wash excess probe from section

4. Cell fixation

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#Question id: 12944

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the following-

A.Tyrosine kinase.             1. Kaposis sacroma

B.HIV Transactivator.      2. Hypertension

C. Angiotensinogen.         3.Atherosclerosis

D.CET protein                      4. Cardiac hypertrophy

TLS Online TPP Program

#Question id: 12945

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

It is a PCR based multiplex assay that allows a number of target sites such as deletions, duplications, mutations to be amplified with a pair of primer containing probes.

TLS Online TPP Program

#Question id: 12946

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Choose the incorrect statement for the Multiple Ligation dependent probe amplification.

TLS Online TPP Program

#Question id: 12947

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the following-

A. G- proteins.                    1.src

B. Growth factors.            2.erbB

C. Protein kinases.           3. N-ras

D. Growth factor receptor.   4.hst