The double-reciprocal transformation of the Michaelis-Menten equation, also called the Lineweaver-Burk plot, is given by To determine Km from a double-reciprocal plot, you would:

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#Question id: 13088

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;

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#Question id: 14678

#Section 4: Fundamentals of Biological Engineering

Pure chlorine enters a process. By measurement it is found that 2.4 kg of chlorine pass into the process every 3.1 min. Calculate the molar flow rate of the chlorine in kilogram moles per hour.

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#Question id: 31208

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

In MSA, what is the significance of highly conserved regions?

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#Question id: 14152

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The statistical analysis of alignment scores is much better understood for ________ than for _______

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#Question id: 4911

#Section 3: Genetics, Cellular and Molecular Biology

Neutral petite mutations: