function of CR2

TLS Online TPP Program

#Question id: 13054

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

TLS Online TPP Program

#Question id: 2065

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following are least likely to diffuse through the phospholipid bilayer of a cell membrane?

TLS Online TPP Program

#Question id: 19530

#Section 4: Fundamentals of Biological Engineering

If air consists of 77% by weight of nitrogen and 23% by weight of oxygen calculate:

(i) the mean molecular weight of air,

(ii) the mole fraction of oxygen,

(iii) the concentration of oxygen in mole/m3 and kg/m3  if the total pressure is 1.5 atmospheres and the temperature is 25 °C.

TLS Online TPP Program

#Question id: 10541

#Section 6: Plant, Animal and Microbial Biotechnology

Green-leaf volatiles act as potent signals,  which of the following option is incorrect about this volatile compound

TLS Online TPP Program

#Question id: 16866

#General Aptitude

He looks upset, I think he took the criticism_____ heart.