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TLS Online TPP Program

#Question id: 3893

Protein structural motifs often have general functions in common.  Which one of the following motifs is known to be involved in protein dimer formation, but not in direct protein-DNA interactions?

#Section 3: Genetics, Cellular and Molecular Biology

  1. b-barrel 

  2. helix-turn-helix

  3. homeodomain

  4. leucine zipper

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TLS Online TPP Program

#Question id: 27272

#Section 5: Bioprocess Engineering and Process Biotechnology

During batch fermentation lowest specific growth rate is achieved during:
TLS Online TPP Program

#Question id: 13091

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
 
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
 
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
                              
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
 
Which colony’s plasmid do you actually want to use for your studies?
TLS Online TPP Program

#Question id: 16436

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The PCR consists of three defined sets of times and temperatures, termed steps
TLS Online TPP Program

#Question id: 11469

#Section 3: Genetics, Cellular and Molecular Biology

plant part not absorb light, which type of plastid it is
TLS Online TPP Program

#Question id: 14309

#Section 5: Bioprocess Engineering and Process Biotechnology

Glucose is converted to ethanol by immobilized yeast cells entrapped in gel beads. The specific rate of ethanol production is: qP = 0.2 g ethanol/g-cell-h. The effectiveness factor for an average bead is 0.8. Each bead contains 50 g/L of cells. The voids volume in the column is 40%. Assume growth is negligible (all glucose is converted into ethanol). The feed flow rate is F = 400 l/h and glucose concentration in the feed is S0i = 150 g glucose/l. The diameter of the column is 1 m and the yield coefficient is about 0.49 g ethanol/g glucose. The column height is 4 m. What is the glucose conversion at the exit of the column?