#Question id: 14296
#Section 5: Bioprocess Engineering and Process Biotechnology
A two-stage chemostat system is used for production of
secondary metabolite. The volume of each reactor is 0.5 m3; the flow
rate of feed is 50 l h- 1. Mycelial growth occurs in the first
reactor; the second reactor is used for product synthesis. The concentration of
substrate in the feed is 10 gl-1. Kinetic and yield parameters for
the organism are:
Assume that product synthesis is negligible in the first
reactor and growth is negligible in the second reactor. Determine cell
concentrations entering the second reactor.
#Question id: 14297
#Section 5: Bioprocess Engineering and Process Biotechnology
A two-stage chemostat system is used for production of
secondary metabolite. The volume of each reactor is 0.5 m3; the flow
rate of feed is 50 l h- 1. Mycelial growth occurs in the first
reactor; the second reactor is used for product synthesis. The concentration of
substrate in the feed is 10 gl-1. Kinetic and yield parameters for
the organism are:
Assume that product synthesis is negligible in the first
reactor and growth is negligible in the second reactor. Determine substrate
concentrations entering the second reactor.
#Question id: 14298
#Section 5: Bioprocess Engineering and Process Biotechnology
A two-stage chemostat system is used for production of
secondary metabolite. The volume of each reactor is 0.5 m3; the flow
rate of feed is 50 l h- 1. Mycelial growth occurs in the first
reactor; the second reactor is used for product synthesis. The concentration of
substrate in the feed is 10 gl-1. Kinetic and yield parameters for
the organism are:
Assume that product synthesis is negligible in the first
reactor and growth is negligible in the second reactor. What is the overall
substrate conversion?
#Question id: 14299
#Section 5: Bioprocess Engineering and Process Biotechnology
Assume that product synthesis is negligible in the first
reactor and growth is negligible in the second reactor. What is the final
concentration of product?
#Question id: 14300
#Section 5: Bioprocess Engineering and Process Biotechnology
A 15-m 3 chemostat is operated with dilution rate 0.1 h-1. A continuous steriliser with steam injection and flash cooling delivers sterilized medium to the fermenter. Medium in the holding section of the steriliser is maintained at 130⁰C The concentration of contaminants in the raw medium is 105 ml-l; an acceptable contamination risk is one organism every 3 months. The Arrhenius constant and activation energy for thermal death are estimated to be 7.5 × 1039 h-1 and 288.5 kJ gmol- 1, respectively. The steriliser pipe inner diameter is 12 cm. At 130⁰C the liquid density is 1000 kg m- 3 and the viscosity is 4 kg m- 1 h- 1. Assuming perfect plug flow, determine the length of the holding section. __________________
#Question id: 14301
#Section 5: Bioprocess Engineering and Process Biotechnology
Pseudomonas sp. has a mass doubling time of
2.4 h when grown on acetate. The saturation constant using this substrate is
1.3 g/l (which is unusually high), and cell yield on acetate is 0.46 g cell/g
acetate. If we operate a chemostat on a feed stream containing 38 g/l acetate,
find the Cell concentration when the dilution rate is one-half of the maximum?