TLS Online TPP Program

#Question id: 4129


Which of the following statements about the tRNA that normally accepts phenylalanine is false?  (mRNA codons for phenylalanine are UUU and UUC.)

#Section 3: Genetics, Cellular and Molecular Biology
  1. It interacts specificially with the Phe synthetase.

  2. It will accept only the amino acid phenylalanine.

  3. Its molecular weight is about 25,000.

  4. The tRNA must contain the sequence UUU.

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TLS Online TPP Program

#Question id: 14190

#Section 5: Bioprocess Engineering and Process Biotechnology

Aerobic degradation of an organic compound by a mixed culture of organisms in waste water can be represented by the following reaction

               

Determine RQ for the organisms

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TLS Online TPP Program

#Question id: 14191

#Section 5: Bioprocess Engineering and Process Biotechnology

Consider a 1000-l CSTR in which biomass is being produced with glucose as the substrate. The microbial system follows a Monod relationship withn µm = 0.4 h-1 , KS = 1.5 g/l (an unusu ally high value), and the yield factor YX/S = 0.5 g biomass/g substrate consumed. If normal operation is with a sterile feed containing 10 g/l glucose at a rate of 100 l/h. What is the specific biomass production rate (g/l-h) at steady state?

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TLS Online TPP Program

#Question id: 14192

#Section 5: Bioprocess Engineering and Process Biotechnology

In a two-stage chemostat system, the volumes of the first and second reactors are V1 = 500 l and V2 = 300 l, respectively. The first reactor is used for biomass production and the second is for a secondary metabolite formation. The feed flow rate to the first reactor is F = 100 l/h, and the glucose concentration in the feed is S = 5.0 g/l. Use the following constants for the cells.



Determine cell concentration in the effluent of the first stage.

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TLS Online TPP Program

#Question id: 14193

#Section 5: Bioprocess Engineering and Process Biotechnology

In a two-stage chemostat system, the volumes of the first and second reactors are V1 = 500 l and V2 = 300 l, respectively. The first reactor is used for biomass production and the second is for a secondary metabolite formation. The feed flow rate to the first reactor is F = 100 l/h, and the glucose concentration in the feed is S = 5.0 g/l. Use the following constants for the cells.



Determine glucose concentration in the effluent of the first stage. _____________________

TLS Online TPP Program

#Question id: 14194

#Section 5: Bioprocess Engineering and Process Biotechnology

Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. Determine the culture volume at t = 10 h

TLS Online TPP Program

#Question id: 14195

#Section 5: Bioprocess Engineering and Process Biotechnology

Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. Determine the concentration of glucose at t = 10 h at quasi-steady state