TLS Online TPP Program

#Question id: 4131


Aminoacyl-tRNA synthetases (amino acid activating enzymes):

#Section 3: Genetics, Cellular and Molecular Biology
  1. “recognize” specific tRNA molecules and specific amino acids.

  2. in conjunction with another enzyme attach the amino acid to the tRNA.

  3. interact directly with free ribosomes.

  4. occur in multiple forms for each amino acid.

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TLS Online TPP Program

#Question id: 14165

#Section 5: Bioprocess Engineering and Process Biotechnology

Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is:

                                    

Assuming 100% conversion, what is the yield of cells from oxygen in g g-l?

TLS Online TPP Program

#Question id: 14166

#Section 4: Fundamentals of Biological Engineering

Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is:

                             

You have been put in charge of a small fermenter for growing the bacteria and aim to produce 2.5 kg of cells for inoculation of a pilot-scale reactor. What minimum amount of hexadecane substrate must be contained in your culture medium? 

TLS Online TPP Program

#Question id: 14166

#Section 5: Bioprocess Engineering and Process Biotechnology

Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is:

                             

You have been put in charge of a small fermenter for growing the bacteria and aim to produce 2.5 kg of cells for inoculation of a pilot-scale reactor. What minimum amount of hexadecane substrate must be contained in your culture medium? 

TLS Online TPP Program

#Question id: 14167

#Section 4: Fundamentals of Biological Engineering

Corn-steep liquor contains 2.5 % invert sugars and 50% water; the rest can be considered solids. Beet molasses containing 50% sucrose, 1% invert sugars, 18% water and the remainder solids, is mixed with corn-steep liquor in a mixing tank. Water is added to produce a diluted sugar mixture containing 2% (w/w) invert sugars. 125 kg corn-steep liquor and 45 kg molasses are fed into the tank.

 How much water is required?


TLS Online TPP Program

#Question id: 14167

#Section 5: Bioprocess Engineering and Process Biotechnology

Corn-steep liquor contains 2.5 % invert sugars and 50% water; the rest can be considered solids. Beet molasses containing 50% sucrose, 1% invert sugars, 18% water and the remainder solids, is mixed with corn-steep liquor in a mixing tank. Water is added to produce a diluted sugar mixture containing 2% (w/w) invert sugars. 125 kg corn-steep liquor and 45 kg molasses are fed into the tank.

 How much water is required?


TLS Online TPP Program

#Question id: 14168

#Section 4: Fundamentals of Biological Engineering

Corn-steep liquor contains 2.5 % invert sugars and 50% water; the rest can be considered solids. Beet molasses containing 50% sucrose, 1% invert sugars, 18% water and the remainder solids, is mixed with corn-steep liquor in a mixing tank. Water is added to produce a diluted sugar mixture containing 2% (w/w) invert sugars. 125 kg corn-steep liquor and 45 kg molasses are fed into the tank.

What is the concentration of solids in the final mixture?