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TLS Online TPP Program
#Question id: 556
#Section 2: General Biology
Which of the following statement is correct to calculate the turnover number of an enzyme,
A) the enzyme concentration.
B) the initial velocity of the catalyzed reaction at [S] >> Km.
C) the initial velocity of the catalyzed reaction at low [S].
D) the Km for the substrate.
TLS Online TPP Program
#Question id: 13060
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
In blue white screening insertional activation of Beta-D-galactosidase will give rise to
TLS Online TPP Program
#Question id: 14421
#Section 1: Engineering Mathematics
TLS Online TPP Program
#Question id: 15674
#Section 6: Plant, Animal and Microbial Biotechnology
During which phase of the bacterial growth curve does the total number of viable cells decline?
TLS Online TPP Program
#Question id: 1314
#Section 3: Genetics, Cellular and Molecular Biology
In desmosomes, cadherins link to an adjacent cell via: