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TLS Online TPP Program
#Question id: 13087
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?
TLS Online TPP Program
#Question id: 1120
#Section 3: Genetics, Cellular and Molecular Biology
A mutation that knocks out the GTPase activity of a G protein would have what effect on a cell?
TLS Online TPP Program
#Question id: 20601
#Section 5: Bioprocess Engineering and Process Biotechnology
The difference between the set points and the measured signal is known as ______________?
TLS Online TPP Program
#Question id: 543
#Section 4: Fundamentals of Biological Engineering
If glucose labeled with 14C at C-1 (the aldehyde carbon) were metabolized in the liver, the first radioactive pyruvate formed would be labeled in:
TLS Online TPP Program
#Question id: 1844
#Section 2: General Biology
Which of the following diseases has been completely eradicated worldwide?