In Burkitt's lymphoma, a vaccine against which of the following might prove useful?

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#Question id: 3315

#Section 3: Genetics, Cellular and Molecular Biology

Which one of the following statements is TRUE for Negative-frequency dependent selection?

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#Question id: 15574

#Section 5: Bioprocess Engineering and Process Biotechnology

For the first-order reaction given by 2N₂O₅ → 4NO₂ + O₂ the initial concentration of N₂O₅ was 0.1M (at a constant temperature of 300K). After 10 minutes, the concentration of N₂O₅ was found to be 0.01M. Find the rate constant of this reaction (at 300K).

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#Question id: 154

#Section 2: General Biology

The peptide bond is which of the following?

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#Question id: 5248

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following is a description of generalized transduction?

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#Question id: 13090

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 3’s plasmid is;