Practice Questions

TLS Online TPP Program

#Question id: 1965

In some insects, such as Drosophila, fungal cell wall elements can activate the protein Toll. What is Tollʹs function?

#Section 2: General Biology

acts as a receptor that, when activated, signals synthesis of antimicrobial peptides
functions directly to attack the fungi presented to it
produces antimicrobial peptides by interaction with chitin
secretes special recognition signal molecules that identifies specific pathogens

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TLS Online TPP Program

#Question id: 13077

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The DNA probes for RFLP may NOT be obtained from

TLS Online TPP Program

#Question id: 13078

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

RFLPs have several unique advantages except this one;

TLS Online TPP Program

#Question id: 13079

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Name of that sophisticated equipment which used to the oligonucleotide, that serve as a primer in RAPDs is usually obtained by in vitro DNA synthesis,

TLS Online TPP Program

#Question id: 13080

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

In RAPDs amplification will take place only of those regions of the genome that have the

TLS Online TPP Program

#Question id: 13081

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Correct statements about RAPD’s

A. RAPD polymorphism is detected by using oligonucleotides usually more than 10 bases long of random sequences as primers in a reaction.

B. In a strain which has in genomic DNA sequences complementary to the primer oligonucleotide, PCR products will be detected in the gel,

C. Typical RAPD markers show limited variation between parents, especially in naturally inbreeding species.

D. RAPDs are more sensitive than RFLPs to experimental conditions making them more difficult to be consistent and reproducible.

TLS Online TPP Program

#Question id: 13082

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Statement: AFLP shares some features of both RFLP and RAPD analyses.

Explanations: I. It uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.

II. AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.