The MHC is important in a T cellʹs ability to

TLS Online TPP Program

#Question id: 13055

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?

TLS Online TPP Program

#Question id: 27432

#Section 5: Bioprocess Engineering and Process Biotechnology

Damkolher number (Noa) is the ratio of

TLS Online TPP Program

#Question id: 14416

#Section 2: General Biology

Bacteriostatic and Bactericidal functions can be achieved by

TLS Online TPP Program

#Question id: 13057

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

TLS Online TPP Program

#Question id: 15599

#Section 1: Engineering Mathematics

Find the positive real root of x³ - x - 3 = 0 using Newton-Raphson method. If the starting guess (x0) is 2, the numerical value of the root after two iterations (x2) is   (round off to two decimal places).