Which of the following is NOT an assumption of the Hardy- Weinberg model?

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#Question id: 16781

#Section 1: Engineering Mathematics

At Dunkin Donuts the number of donut holes in a bag can vary. Help Donald find the mode. 12,10,10,10,13,12,11,13,10

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#Question id: 15513

#Section 1: Engineering Mathematics

Which is the correct expression for the complementary function solution of

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#Question id: 15560

#Section 6: Plant, Animal and Microbial Biotechnology

Nearly all of the genetic improvements to industrially important antibiotic-producing strains have been achieved by the use of 

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#Question id: 1668

#Section 2: General Biology

What does the cytoplasmic region of cell surface IgM consists of?

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#Question id: 13092

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?