Repulsion and coupling are two faces of

TLS Online TPP Program

#Question id: 13092

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

TLS Online TPP Program

#Question id: 14184

#Section 5: Bioprocess Engineering and Process Biotechnology

Aerobic degradation of benzoic acid by a mixed culture of microorganisms can be represented by the following reaction.

             Determine degree of reduction for bacteria.

______________________

TLS Online TPP Program

#Question id: 13049

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

What is the role of goat anti-rabbit IgG horseradish peroxidase conjugate in the experiment?

TLS Online TPP Program

#Question id: 4910

#Section 3: Genetics, Cellular and Molecular Biology

For a trait known to be caused by a mitochondrial gene variant, there may be an occasional female that can transmit different phenotypes to her progeny on an apparently random basis. What situation might you suspect?

TLS Online TPP Program

#Question id: 3315

#Section 3: Genetics, Cellular and Molecular Biology

Which one of the following statements is TRUE for Negative-frequency dependent selection?