Auxotrohic bacteria plating on different medium, it is observed that colonies grow on minimal plus threonine only, no colony on minimal plus leucine and no colonies grow on minimal medium cystein only. What are the actual genotypes of auxotroph?

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#Question id: 14625

#Section 5: Bioprocess Engineering and Process Biotechnology

Monod kinetics are considered in a CSTR with an organism growing with an initial substrate concentration of 50 g1^-1 and kinetic parameters of Ks = 2 g1^-1 and umax = 0.5 h^-1 . (a) What would be the maximum dilution rate for 100% yield of biomass with maximum rate? 

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#Question id: 14626

#Section 5: Bioprocess Engineering and Process Biotechnology

A bacterial fermentation was carried out in a reactor containing broth with average density 1200 kg/m³ and viscosity 0.02 N.s/m² . The broth was agitated at 90 rpm and air was introduced through the sparger at a flow rate of 0.4 vvm. The fermenter was equipped with two sets of flat blade turbine impellers and four baffles. The dimensions of vessel, impellers and baffle width were: tank diameter, Dt =4 m; impeller diameter, Di = 2 m; baffle width, Wb = 0.4 m; also, the liquid depth was H = 6.5 m 

Determine ungassed power, P; 

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#Question id: 14627

#Section 5: Bioprocess Engineering and Process Biotechnology

In a batch production of penicillin, 40 m³ capacity of the plant, sterile air is required to be supplied. The bioreactor requires 1 vvm (volume of air/volume of broth. min). The incoming air contains 3000 cells/m³of air, for 100 hours operation. Calculate the depth of filter if the penetration of bacteria is 1 in 1 million. 

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#Question id: 14628

#Section 5: Bioprocess Engineering and Process Biotechnology

Microbial cells are separated from a culture broth at a flow rate of 3.35 x10^-3m3/5. Assume the cells are spherical with average diameter of 111m. Select a centrifuge that can perform this separation. Given data: p_cell =1.1, P_water,=997 kgm^-1 at 25 °C; P_broth = 3p_water, and the viscosity of water is 0.9 x 10^-3 N.s/m² 

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#Question id: 14629

#Section 5: Bioprocess Engineering and Process Biotechnology

Microbial cell recovery is carried out in a continuous disc-stack centrifuge. The centrifuge is operated at 5000 rpm for separation of baker's yeast. At a feed rate of 60 1/min, 50% of the cells are recovered. At constant speed, solid recovery is inversely proportional to flow rate. What flow rate is required to recover 90% of cells if the centrifuge speed is fixed at 5000 rpm? 

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#Question id: 14630

#Section 1: Engineering Mathematics

The series whose n^th term is sin 1/n is