To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

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#Question id: 3029

#Section 2: General Biology

Which of the following obtain energy by oxidizing inorganic substances; energy that is used, in part, to fix CO2?

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#Question id: 3682

#Section 3: Genetics, Cellular and Molecular Biology

A mutated strain of E. coli replicates DNA normally at 25°C, but replication stops immediately when the temperature is raised to 37°C. Which protein is likely to be non-functional at the higher temperature?

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#Question id: 3538

#Section 3: Genetics, Cellular and Molecular Biology

Monohybrid cross allowed between heterozygous Rr (Round) and RR (Round), all progeny having Round seeded plant were allowed to self-fertilize to produce numerous progeny that are 

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#Question id: 1698

#Section 2: General Biology

An example of a “non-classical” MHC molecule is:

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#Question id: 21160

#Section 5: Bioprocess Engineering and Process Biotechnology

If the time required to change the concentration of reactant to half its original value is independent of the initial concentration, the order of reaction is