To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 2’s plasmid is;

#Question id: 4830

#Section 3: Genetics, Cellular and Molecular Biology

Blue flower is dominant over white flower and long pollen is dominant over short pollen. A pure line plant with Blue flower and long pollen was cross with white flower and short pollen. F1 plant was allowed to test cross to produced numerous Offspring in following number

Blue flower and long pollen            2000

Blue flower and short pollen             500

White flower and long pollen            500    

White flower and short pollen         2000

What is approximate distance between Flower color gene and pollen length gene?

#Question id: 4831

#Section 3: Genetics, Cellular and Molecular Biology

There are 4 pairs of chromosomes in a Drosophila.The linkage groups present in it are

#Question id: 4832

#Section 3: Genetics, Cellular and Molecular Biology

Percentage of crossing over is more when

#Question id: 4833

#Section 3: Genetics, Cellular and Molecular Biology

If the percentage of chance of crossing over between two genes is10, then the distance between two genes will be

#Question id: 4834

#Section 3: Genetics, Cellular and Molecular Biology

Alleles of different genes that are on the same chromosome can occasionally be separated by a phenomenon called

#Question id: 4835

#Section 3: Genetics, Cellular and Molecular Biology

Repulsion and coupling are two faces of