To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

#Question id: 3579

#Section 3: Genetics, Cellular and Molecular Biology

Following some statement is correct with monohybrid and Dihybrid cross?

A) Two alleles for gene segregate into gametes in equal proportions.

B) Monohybrid F2 phenotype 1:2:1 ratio conclude, there is no segregation in character

C)  Monohybrid F2 phenotype 3:1 ratio conclude, there is a no blending inheritance

D) Dihybrid F 2 phenotype 3:1 ratio conclude complete linkage

#Question id: 3580

#Section 2: General Biology

Different genetic cross regarding two independently assorting non allelic gene with complete dominance and their phenotypic ratio showed in a following. Which of the following is incorrect combination? 

#Question id: 3580

#Section 3: Genetics, Cellular and Molecular Biology

Different genetic cross regarding two independently assorting non allelic gene with complete dominance and their phenotypic ratio showed in a following. Which of the following is incorrect combination? 

#Question id: 3581

#Section 2: General Biology

What is the probability that each of the following pairs of parents will produce the indicated offspring? (Assume independent assortment of all gene pairs.)

(a) AABBCc X  aabbcc → AaBbCc          (b) AABbCc  X AaBbCc → AAbbCC

(c) AaBbCc  X AaBbCc → AaBbCc         (d) aaBbCC  X  AABbcc → AaBbCc

#Question id: 3581

#Section 3: Genetics, Cellular and Molecular Biology

What is the probability that each of the following pairs of parents will produce the indicated offspring? (Assume independent assortment of all gene pairs.)

(a) AABBCc X  aabbcc → AaBbCc          (b) AABbCc  X AaBbCc → AAbbCC

(c) AaBbCc  X AaBbCc → AaBbCc         (d) aaBbCC  X  AABbcc → AaBbCc

#Question id: 3582

#Section 2: General Biology

Different genetic cross regarding two independently assorting non allelic gene with complete dominance and their phenotypic ratio showed in a following is correc