Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

#Question id: 4807

#Section 3: Genetics, Cellular and Molecular Biology

Three incompletely link marker gene was found. There is a marker A gene present in middle. Cross between aaBBcc X AAbbCC to produce heterozygous were allowed test cross, which of the following test cross offspring should be least frequent?

#Question id: 4808

#Section 3: Genetics, Cellular and Molecular Biology

When F1 female Drosophila of the genotype AaBbCc is test crossed, ( Gene A and B located at 10 map unit in transe configuration but gene C are independently assort for both gene. Which of the following test cross progeny that is that is more frequent?

#Question id: 4809

#Section 3: Genetics, Cellular and Molecular Biology

The upper most following figure above shows the locations of four genes on the genetic map of an organism; the lower figure shows the locations of the same four genes on a physical map derived from the nucleotide sequence of the DNA of that organism. The maps are not identical because

#Question id: 4810

#Section 3: Genetics, Cellular and Molecular Biology

In a haploid organism, the C and D loci are 8 m.u. apart. From a cross C d X c D, which of the following proportion following progeny classes: (a) C D; (b) c d; (c) C d

#Question id: 4811

#Section 3: Genetics, Cellular and Molecular Biology

Three incompletely link gene as a B-A-C order. A cross allowed between AAbbcc X aaBBCC produce f1 progeny allowed test cross. Which of the following offspring is a result of double cross over?

#Question id: 4812

#Section 3: Genetics, Cellular and Molecular Biology

Two character X and Y ,both follow law of dominance due to 3:1 F2 phenotypic ratio monohybrid cross separately If carry out dihybrid selfing cross for heterozygous genotype for both character does not follow law of independence assortment in normal environmental condition due to?