Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

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#Question id: 15901

#Section 6: Plant, Animal and Microbial Biotechnology

The following features are included between the shoot buds and somatic embryo;

                                                                             FEATURES

A) SHOOT BUD                                 i) Originated from the many cells, usually superficial

B) SOMATIC EMBRYO                        ii) Polarity is defined as bipolar; both shoot and root poles present

                                                        iii) Vascular strands connected with those present in callus/explant

                                                       iv) Easily separated from the callus/explant

                                                        v) There is no vascular connection with callus/explant

Which of the following correctly represents the features of  shoot buds and somatic embryo?

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#Question id: 15902

#Section 6: Plant, Animal and Microbial Biotechnology

The pair of hormones required for a callus to differentiate are____

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#Question id: 15903

#Section 6: Plant, Animal and Microbial Biotechnology

Protoplast can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with

TLS Online TPP Program

#Question id: 15904

#Section 6: Plant, Animal and Microbial Biotechnology

If the cell arc treated with 0.01% solution of FDA, what will be the effect on live cell?

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#Question id: 15904

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

If the cell arc treated with 0.01% solution of FDA, what will be the effect on live cell?

TLS Online TPP Program

#Question id: 15905

#Section 6: Plant, Animal and Microbial Biotechnology

Meristemoids are considered to arise in areas that accumulate polysaccharide, which is believed to serve as an energy source for shoot bud differentiation